It is possible that the transgenic founder may be infertile due to consequences of transgene expression or unknown reasons. If no litters are produced after one month you should replace the mice that you are mating with your founder. Expect litters within a month of mating since female mice go into estrus every 3 or 4 days and the gestation time of mice is 19-21 days. This will produce mice with the targeted gene mutation on the 129/Sv+Tyr-c+p background for comparison with the mutation on the C57BL/6 background.ģ. Alternatively, chimeras can be mated with 129/Sv+Tyr-c+p mice, which have the same genotype as the embryonic stem cells. By analyzing gene expression on a C57BL/6 background, any influence of the genetic background on gene expression will be controlled for by comparison to normal C57BL/6 mice. After 6 generations of mating to C57BL/6, more than 99% of the genetic background will be C57BL/6. We recommend that transgenic founders or chimeras be mated to C57BL/6 mice. Mate mice when they are sexually mature (6 to 8 weeks old). Make a pedigree for each transgenic founder or embryonic stem cell-mouse chimera.Ģ. They are open to modification and should not be construed as a comprehensive set of rules. These suggestions are based on our experience. We hope that this information will be useful to investigators who are unfamiliar with mouse breeding or who are breeding transgenic or gene targeted mice for the first time.
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